Application
ChIP was performed with the nuclear extract of mouse macrophage (derived from bone marrow) and either 1 μg of PPARG antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to PDK4 PPAR response element (RE).Application
WB analysis of 293T cells transfected with pNTAP-PPARG (lane 1) and non-transfected cells (lane 2) using PPARG antibody diluted 1:2,000 in TBS-Tween containing 3% skimmed milk.Application
ELISA was performed using a serial dilution of PPARG antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:70,250.| Product Name | PPAR gamma antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PPAR gamma |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWBChIP |
| ELISA (1:1,000), Western blotting (1:2,000), ChIP (1 μg/ChIP): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GLM1 NR1C3 PPARgamma PPARG1 peroxisome proliferator-activated receptor gamma CIMT1 PPARG2 |
|---|---|
| Target | PPARG |
Application
ChIP was performed with the nuclear extract of mouse macrophage (derived from bone marrow) and either 1 μg of PPARG antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to PDK4 PPAR response element (RE).
Application
WB analysis of 293T cells transfected with pNTAP-PPARG (lane 1) and non-transfected cells (lane 2) using PPARG antibody diluted 1:2,000 in TBS-Tween containing 3% skimmed milk.
Application
ELISA was performed using a serial dilution of PPARG antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:70,250.| Application Notes | ELISA (1:1,000), Western blotting (1:2,000), ChIP (1 μg/ChIP): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
