Application
Immunofluorescence analysis of methanol-fixed HeLa, using PPAT antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE diluted at 1:1000| Product Name | PPAT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPAT (amidophosphoribosyltransferase)The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPAT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (94%), Rat (91%), Rhesus Monkey (100%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATASE antibody GPAT antibody PRAT antibody PPAT antibody amidophosphoribosyltransferase antibody glutamine phosphoribosylpyrophosphatate amidotransferase antibody glutamine phosphoribosylpyrophosphate amidotransferase antibody glutamine PRPP amidotr |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PPAT antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE diluted at 1:1000| Positive Control | HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
