Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPIH antibody at 1:200 dilution.Application
PPIH antibody detects PPIH protein at nucleus on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. PPIH antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000| Product Name | PPIH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPIH (peptidylprolyl isomerase H (cyclophilin H))The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPIH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Cat, Chicken, Rhesus Monkey, BovineMouse (100%), Zebrafish (88%), Xenopus laevis (92%), Cat (100%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYP-20 antibody CYPH antibody MGC5016 antibody SnuCyp-20 antibody USA-CYP antibody PPIH antibody cyclophilin H antibody peptidyl-prolyl cis-trans isomerase H antibody PPIase h antibody rotamase H antibody U-snRNP-associated cyclophilin SnuCyp-20 |
|---|---|
| Molecular Weight(MW) | 19 kDa (note) (2) |
| Cellular Localization | Nucleus speckle , Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPIH antibody at 1:200 dilution.
Application
PPIH antibody detects PPIH protein at nucleus on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. PPIH antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
