Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPIL4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 C: HCT116 7.5% SDS PAGE diluted at 1:1000| Product Name | PPIL4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPIL4 (peptidylprolyl isomerase (cyclophilin)-like 4)This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPIL4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Dog, Rhesus Monkey, BovineMouse (83%), Dog (93%), Rhesus Monkey (96%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HDCME13P antibody PPIL4 antibody PPIase antibody peptidyl-prolyl cis-trans isomerase-like 4 antibody cyclophilin-like protein PPIL4 antibody rotamase PPIL4 antibody cyclophilin-type peptidyl-prolyl cis-trans isomerase antibody serologically defined |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:26991011) |
| Cellular Localization | Nucleus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPIL4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 C: HCT116 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
