Application
ELISA analysis of control antigen and antigen using PPM1A antibody [7F12].Application
FACS analysis of HeLa cells using PPM1A antibody [7F12] (green) or a negative control (red).Application
WB analysis of lysates from (1) untransfected and (2) PPM1A (a.a. 202-382)-hIgGFc transfected HEK293 cells using PPM1A antibody [7F12].Application
WB analysis of (1) Jurkat, (2) Jurkat, (3) A431, (4) HeLa, (5) HEK293, (6) Raji, (7) MCF-7 and (8) COS7 lysates using PPM1A antibody [7F12].Application
WB analysis of human PPM1A recombinant protein using PPM1A antibody [7F12].| Product Name | PPM1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [7F12] to PPM1AThe protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 202 and 382 of human PPM1A expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F12 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | PP2CA PP2CALPHA PP2C-ALPHA |
|---|---|
| Target | PPM1A |
| Molecular Weight(MW) | 42.4 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PPM1A antibody [7F12].
Application
FACS analysis of HeLa cells using PPM1A antibody [7F12] (green) or a negative control (red).
Application
WB analysis of lysates from (1) untransfected and (2) PPM1A (a.a. 202-382)-hIgGFc transfected HEK293 cells using PPM1A antibody [7F12].
Application
WB analysis of (1) Jurkat, (2) Jurkat, (3) A431, (4) HeLa, (5) HEK293, (6) Raji, (7) MCF-7 and (8) COS7 lysates using PPM1A antibody [7F12].
Application
WB analysis of human PPM1A recombinant protein using PPM1A antibody [7F12].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
