Application
WB analysis of mouse liver cytosol extract using PP2C alpha antibody at a dilution of 1:250.| Product Name | PPM1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [p6c7] to PPM1AThe protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human PP2C alpha (1-382aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | p6c7 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PP2CA PPM1A protein phosphatase Mg2+/Mn2+ dependent 1A PP2C-ALPHA |
|---|---|
| Target | PPM1A |
| Light Chain Type | kappa |
Application
WB analysis of mouse liver cytosol extract using PP2C alpha antibody at a dilution of 1:250.| Application Notes | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
