Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PPM1F Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinomaApplication
Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35ug/lane). PPM1F (arrow) was detected using the purified Pab.Application
Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2).| Product Name | PPM1F antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PPM1FPPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. |
| Immunogen | KLH conjugated synthetic peptide between 18~48 amino acids from the N-terminal region of human PPM1F |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For ELISA: Use at a 1:1,000 dilution. For WB: Use at a 1:100-1:500 dilution. For IHC-P: Use at a 1:50-1:100 dilution.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Ca(2+)/calmodulin-dependent protein kinase phosphatase CaM-kinase phosphatase CaMKPase Partner of PIX 2 hFEM-2 Protein phosphatase 1F POPX2 |
|---|---|
| Target | PPM1F |
| Molecular Weight(MW) | 49.8 (note) |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PPM1F Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma
Application
Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35ug/lane). PPM1F (arrow) was detected using the purified Pab.
Application
Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2).| Application Notes | For ELISA: Use at a 1:1,000 dilution. For WB: Use at a 1:100-1:500 dilution. For IHC-P: Use at a 1:50-1:100 dilution.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
