Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PPME1 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.Application
HEK293T cells transfected with either pCMV6-ENTRY PPME1Application
Anti-PPME1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PPME1| Product Name | PPME1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10A12] to PPME1Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro). |
| Immunogen | Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 10A12 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PME 1 PME1 |
|---|---|
| Target | PPME1 |
| Tissue Specificity | is specific for human PPME1 (NP_057231). |
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PPME1 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
Application
HEK293T cells transfected with either pCMV6-ENTRY PPME1
Application
Anti-PPME1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PPME1| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
