Application
Sample (30 ug of whole cell lysate) A: A549 B: HCT116 10% SDS PAGE diluted at 1:2000| Product Name | PPOX antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPOX (protoporphyrinogen oxidase)This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPOX. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (83%), Rat (81%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC8485 antibody PPO antibody V290M antibody VP antibody PPOX antibody protoporphyrinogen oxidase antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (2) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side |
Application
Sample (30 ug of whole cell lysate) A: A549 B: HCT116 10% SDS PAGE diluted at 1:2000| Positive Control | A549 , HeLa , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
