Application
Western blot analysis using 4E - BP1 monoclonal antibody against truncated 4E - BP1 recombinant protein and A431 cell lysate.| Product Name | 4E-BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphorylated heat- and acid-stable protein regulated by insulin 1, 4E-BP1, BP-1, 4EBP1, PHAS-I |
| Product description | 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.1) Pause, A. et al. 1994.Nature. 371:762–767. |
| Immunogen | Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:1,000. ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | EIF4EBP1 |
|---|---|
| Alternative Names | Phosphorylated heat- and acid-stable protein regulated by insulin 1 4E-BP1 BP-1 4EBP1 PHAS-I |
Application
Western blot analysis using 4E - BP1 monoclonal antibody against truncated 4E - BP1 recombinant protein and A431 cell lysate.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:1,000. ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | 4E-BP1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
