Application
Immunofluorescence analysis of methanol-fixed HeLa, using PPP2R1A antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PPP2R1A antibody detects PPP2R1A protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PPP2R1A antibody diluted at 1:2500.| Product Name | PPP2R1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPP2R1A (serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform)This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Rhesus Monkey, BovineRat (100%), Zebrafish (88%), Xenopus laevis (95%), Pig (100%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC786 antibody PP2A-Aalpha antibody PP2AAALPHA antibody PR65A antibody PPP2R1A antibody PP2A subunit A isoform PR65-alpha antibody medium tumor antigen-associated 61 KDA protein antibody serine/threonine-protein phosphatase 2A 65 kDa regulatory sub |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PPP2R1A antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PPP2R1A antibody detects PPP2R1A protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PPP2R1A antibody diluted at 1:2500.| Positive Control | HeLa , NIH-3T3 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
