Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | 5HT3E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5-hydroxytryptamine receptor 3E, 5-HT3-E, 5-HT3E, Serotonin receptor 3E, HTR3E |
| Product description | The product of this gene belongs to the ligand-gated ion channel receptor superfamily. HTR3E encodes a subunit E of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. Genes encoding subunits C, D and E form a cluster on chromosome 3. An alternative splice variant has been described but its full length sequence has not been determined.1) Walstab, J., et al. J. Biol. Chem. 285(35):26956-26965(2010) |
| Immunogen | This 5HT3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-154 amino acids from the Central region of human 5HT3E. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HTR3E |
|---|---|
| Alternative Names | 5-hydroxytryptamine receptor 3E 5-HT3-E 5-HT3E Serotonin receptor 3E HTR3E |
| Molecular Weight(MW) | 51 kDa |
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
