Application
ELISA analysis of control antigen and antigen using PPP2R4 antibody [4D9].Application
FACS analysis of MCF-7 cells using PPP2R4 antibody [4D9] (green) or a negative control (red).Application
ICC/IF analysis of HeLa cells using PPP2R4 antibody [4D9] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of cervical cancer tissues using PPP2R4 antibody [4D9].Application
IHC-P analysis of lung cancer tissues using PPP2R4 antibody [4D9].Application
WB analysis of human PPP2R4 (a.a. 1-154) recombinant protein using PPP2R4 antibody [4D9].| Product Name | PPP2R4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [4D9] to PPP2R4Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human PPP2R4 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4D9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | PR53 PP2A PTPA |
|---|---|
| Target | PPP2R4 |
| Molecular Weight(MW) | 41 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PPP2R4 antibody [4D9].
Application
FACS analysis of MCF-7 cells using PPP2R4 antibody [4D9] (green) or a negative control (red).
Application
ICC/IF analysis of HeLa cells using PPP2R4 antibody [4D9] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of cervical cancer tissues using PPP2R4 antibody [4D9].
Application
IHC-P analysis of lung cancer tissues using PPP2R4 antibody [4D9].
Application
WB analysis of human PPP2R4 (a.a. 1-154) recombinant protein using PPP2R4 antibody [4D9].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
