Application
PPP3CB antibody detects PPP3CB protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PPP3CB protein stained by PPP3CB antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PPP3CB antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg whole cell lysate) A:A431 B:MOLT4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PPP3CB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPP3CB (protein phosphatase 3, catalytic subunit, beta isozyme) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP3CB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Rabbit, Chicken, BovineRat (100%), Zebrafish (94%), Xenopus laevis (90%), Rabbit (100%), Chicken (97%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CALNA2 antibody CALNB antibody CNA2 antibody PP2Bbeta antibody PPP3CB antibody protein phosphatase from PCR fragment H32 antibody calmodulin-dependent calcineurin A subunit beta isoform antibody protein phosphatase 2B catalytic subunit beta isofor |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (4) |
Application
PPP3CB antibody detects PPP3CB protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PPP3CB protein stained by PPP3CB antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PPP3CB antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg whole cell lysate) A:A431 B:MOLT4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , Molt-4 , mouse brain , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
