Application
HEK293T cells transfected with either RC201650 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP5C antibody, and then analyzed by flow cytometry.Application
Immunofluorescent staining of HT29 cells using anti-PPP5C mouse monoclonal antibody .Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP5C monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PPP5C mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-PPP5C mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PPP5C mouse monoclonal antibody.| Product Name | PPP5C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2E12] to PPP5CMay play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1. |
| Immunogen | Protein expressed in 293T cell transfected with human PPP5C expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2E12 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PPP5C PP5 PPP5 |
|---|---|
| Target | PPP5C |
| Tissue Specificity | is specific for human PPP5C. |
| Cellular Localization | Predominantly nuclear. But also present in the cytoplasm. |
Application
HEK293T cells transfected with either RC201650 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP5C antibody, and then analyzed by flow cytometry.
Application
Immunofluorescent staining of HT29 cells using anti-PPP5C mouse monoclonal antibody .
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP5C monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.
Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PPP5C mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-PPP5C mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PPP5C mouse monoclonal antibody.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
