Application
Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 μg/mL.Application
Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/mL.Application
Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | AATF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AATF Antibody: DED, BFR2, CHE1, CHE-1, DED, HSPC277, Protein AATF, Apoptosis-antagonizing transcription factor |
| Product description | AATF Antibody: AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases. AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways. Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the beta-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer's disease (AD), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.1) AATF antibody can be stored at 4˚C, stable for one year. Page G, Lodige I, Kogel D et al. AATF, a novel transcription factor that interacts with Dlk/ZIP kinase and interferes with apoptosis. FEBS Lett. 1999; 462:187-191. |
| Immunogen | AATF antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human AATF. The immunogen is located within the last 50 amino acids of AATF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AATF antibody can be used for the detection of AATF by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AATF |
|---|---|
| Alternative Names | AATF Antibody: DED BFR2 CHE1 CHE-1 DED HSPC277 Protein AATF Apoptosis-antagonizing transcription factor |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
Application
Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 μg/mL.
Application
Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/mL.
Application
Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | AATF antibody can be used for the detection of AATF by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AATF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AATF Antibody is supplied in PBS containing 0.02% sodium azide. |
