Application
Sample (30 µg of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PPT1 antibody at 1:500 dilution.Application
PPT1 antibody [N1C3] detects PPT1 protein at lysosome on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. PPT1 antibody [N1C3] dilution: 1:500. Scale bar = M_2 μm.Application
PPT1 antibody [N1C3] detects PPT1 protein at lysosome on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PPT1 antibody [N1C3] dilution: 1:500.Application
PPT1 antibody [N1C3] detects PPT1 protein at lysosome on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. PPT1 antibody [N1C3] dilution: 1:500. Scale bar = M μm.| Product Name | PPT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PPT1 (palmitoyl-protein thioesterase 1)The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rhesus Monkey, BovineDog (94%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CLN1 antibody INCL antibody PPT antibody PPT1 antibody PPT-1 antibody ceroid-palmitoyl-palmitoyl-protein thioesterase 1 antibody palmitoyl-protein thioesterase 1 antibody palmitoyl-protein hydrolase 1 antibody |
|---|---|
| Target | PPT1 |
| Molecular Weight(MW) | 34 kDa (note) |
| Cellular Localization | Lysosome |
Application
Sample (30 µg of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PPT1 antibody at 1:500 dilution.
Application
PPT1 antibody [N1C3] detects PPT1 protein at lysosome on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. PPT1 antibody [N1C3] dilution: 1:500. Scale bar = M_2 μm.
Application
PPT1 antibody [N1C3] detects PPT1 protein at lysosome on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PPT1 antibody [N1C3] dilution: 1:500.
Application
PPT1 antibody [N1C3] detects PPT1 protein at lysosome on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. PPT1 antibody [N1C3] dilution: 1:500. Scale bar = M μm.| Positive Control | 293T , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
