Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPWD1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:2000Application
Sample (50 ug of whole cell lysate) A: Rat bone marrow 7.5% SDS PAGE diluted at 1:1000| Product Name | PPWD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPWD1 (peptidylprolyl isomerase domain and WD repeat containing 1)Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPWD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Pig, Rhesus Monkey, BovineMouse (97%), Xenopus laevis (81%), Pig (97%), Rhesus Monkey (100%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0073 antibody PPWD1 antibody peptidylprolyl isomerase domain and WD repeat-containing protein 1 antibody spliceosome-associated cyclophilin antibody peptidylprolyl isomerase domain and WD repeat containing 1 antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (2) |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPWD1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:2000
Application
Sample (50 ug of whole cell lysate) A: Rat bone marrow 7.5% SDS PAGE diluted at 1:1000| Positive Control | K562 , THP-1 , Rat bone marrow |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
