Application
Anti-PPWD1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PPWD1Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPWD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPWD1.Application
HEK293T cells transfected with either RC204570 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPWD1 antibody, and then analyzed by flow cytometry.| Product Name | PPWD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1B8] to PPWD1Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. |
| Immunogen | Full length human recombinant protein of human PPWD1 (NP_056157) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100,: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0073 |
|---|---|
| Target | PPWD1 |
| Tissue Specificity | is specific for human PPWD1 (NP_056157). |
| Cellular Localization | Associated with spliceosomal complexes. |
Application
Anti-PPWD1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PPWD1
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPWD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPWD1.
Application
HEK293T cells transfected with either RC204570 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPWD1 antibody, and then analyzed by flow cytometry.| Application Notes | ICC/IF 1:100,: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
