Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.| Product Name | ABAT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.1) Inada, T., (2008) Pharmacogenet. Genomics 18 (4), 317-323. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ABAT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ABAT antibody can be used for detection of ABAT by ELISA at 1:12500. ABAT antibody can be used for detection of ABAT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABAT |
|---|---|
| Alternative Names | ABAT GABA-AT GABAT NPD009 |
| Molecular Weight(MW) | 55 kDa |
Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.| Application Notes | ABAT antibody can be used for detection of ABAT by ELISA at 1:12500. ABAT antibody can be used for detection of ABAT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
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| Form | Liquid |
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| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ABAT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
