Application
Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5μg/ml.Application
Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 μg/mL.Application
Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 μg/mL.Application
Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 μg/ml.Application
Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 μg/mL.| Product Name | ABCA7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABCA7 Antibody: ABCX, ABCA-SSN, ATP-binding cassette sub-family A member 7 |
| Product description | ABCA7 Antibody: ATP-binding cassette (ABC) transporters are an evolutionarily conserved family that use ATP hydrolysis to catalyze the transport of various molecules across cell membranes. They are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCA7, a member of the ABC1 subfamily, is a full-size ABC transporter consisting of two sets of the multiple membrane-spanning domains plus the Walker motifs for the ATP interaction. ABCA7 shows the highest homology to ABCA1, an essential molecule for cholesterol homeostasis. The high expression levels of ABCA7 in peripheral leukocytes, thymus, spleen and bone marrow suggests a role in the immune system lipid homeostasis.1) Kaminski WE, Orsó E, Diederich W, et al. Identification of a novel human sterol-sensitive ATP-binding cassette transporter (ABCA7). Biochem. Biophys. Res. Commun. 2000; 273:532-8. |
| Immunogen | ABCA7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ABCA7. The immunogen is located within amino acids 130 - 180 of ABCA7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| ABCA7 antibody can be used for detection of ABCA7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCA7 |
|---|---|
| Alternative Names | ABCA7 Antibody: ABCX ABCA-SSN ATP-binding cassette sub-family A member 7 |
| Molecular Weight(MW) | Predicted: 236 kDa Observed: 240 kDa |
| Sequence Similarities | 3) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | ABCA7 antibody is predicted to not cross-react with other ABC1 subfamily proteins. |
Application
Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5μg/ml.
Application
Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 μg/mL.
Application
Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 μg/mL.
Application
Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 μg/ml.
Application
Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | ABCA7 antibody can be used for detection of ABCA7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ABCA7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ABCA7 Antibody is supplied in PBS containing 0.02% sodium azide. |
