Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.Application
ABCC3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ABCC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Canalicular multispecific organic anion transporter 2, ATP-binding cassette sub-family C member 3, Multi-specific organic anion transporter D, MOAT-D, Multidrug resistance-associated protein 3, ABCC3, CMOAT2, MLP2, MRP3 |
| Product description | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This ABCC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-925 amino acids from the Central region of human ABCC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCC3 |
|---|---|
| Alternative Names | Canalicular multispecific organic anion transporter 2 ATP-binding cassette sub-family C member 3 Multi-specific organic anion transporter D MOAT-D Multidrug resistance-associated protein 3 ABCC3 CMOAT2 MLP2 MRP3 |
| Molecular Weight(MW) | 169 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
Application
ABCC3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
