Application
Western blot analysis of ABCF1 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)Application
ABCF1 Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of ABCF1 Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | ABCF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein, ABCF1, ABC50 |
| Product description | The protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process.1) Barcellos,L.F., et.al., PLoS Genet.(10), E1000696 (2009) Paytubi,S., et.al., J. Biol. Chem. 284 (36), 24061-24073 (2009) Saito,A., et.al., J. Hum. Genet. 54 (6), 317-323 (2009) |
| Immunogen | This ABCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 687-716 amino acids from the C-terminal region of human ABCF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCF1 |
|---|---|
| Alternative Names | ATP-binding cassette sub-family F member 1 ATP-binding cassette 50 TNF-alpha-stimulated ABC protein ABCF1 ABC50 |
| Molecular Weight(MW) | 96 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Western blot analysis of ABCF1 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
Application
ABCF1 Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of ABCF1 Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
