Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).Application
ABHD4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ABHD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Abhydrolase domain-containing protein 4, 311-, Alpha/beta-hydrolase 4, Lyso-N-acylphosphatidylethanolamine lipase, ABHD4 |
| Product description | Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity).1) Simon, G.M., et al. J. Biol. Chem. 281(36):26465-26472(2006) |
| Immunogen | This ABHD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-127 amino acids from the Central region of human ABHD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABHD4 |
|---|---|
| Alternative Names | Abhydrolase domain-containing protein 4 311- Alpha/beta-hydrolase 4 Lyso-N-acylphosphatidylethanolamine lipase ABHD4 |
| Molecular Weight(MW) | 39 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Application
ABHD4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
