Application
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in A2058,T47D cell line lysates (35ug/lane).Application
ABHEB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ABHEB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha/beta hydrolase domain-containing protein 14B, Abhydrolase domain-containing protein 14B, 3---, CCG1-interacting factor B, ABHD14B, CIB |
| Product description | ABHEB has hydrolase activity towards p-nitrophenyl butyrate (in vitro). It may activate transcription.1) Padmanabhan, B., et al. Acta Crystallogr.Biol. Crystallogr. 56 (PT 11), 1479-1481 (2000) |
| Immunogen | This ABHEB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the C-terminal region of human ABHEB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABHD14B |
|---|---|
| Alternative Names | Alpha/beta hydrolase domain-containing protein 14B Abhydrolase domain-containing protein 14B 3--- CCG1-interacting factor B ABHD14B CIB |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in A2058,T47D cell line lysates (35ug/lane).
Application
ABHEB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
