Application
Western blot analysis of lysed extracts from HL60 cells using ABL1/2 (phospho-Tyr393/429).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2 (Phospho-Tyr393/429). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/2 (phospho-Tyr393/429).| Product Name | ABL1 / 2 (phospho Tyr393 / Tyr429) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABL, JTK7, p150, c-ABL, v-abl, c-ABL1, bcr/abl, BHLHE39, Abelson murine leukemia viral oncogene homolog 1 |
| Product description | Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.1) Wang, J. Y. et al. (2000) Oncogene 19, 5643-5650. Danial, N. N. et al. (2000) Oncogene 19, 2523-2531. Brasher, B. B. et al. (2000) J. Biol. Chem. 275, 35631-35637. Pluk, H. et al. (2002) Cell 108, 247-259. |
| Immunogen | ABL1/2 (phospho-Tyr393/429) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 393/429 (D-T-Y (p) -T-A) derived from Human ABL1/2. |
| Modification | p-Tyr393 / Tyr429 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABL1 ABL2 |
|---|---|
| Alternative Names | ABL JTK7 p150 c-ABL v-abl c-ABL1 bcr/abl BHLHE39 Abelson murine leukemia viral oncogene homolog 1 |
| Molecular Weight(MW) | 210 kDa |
| Tissue Specificity | This antibody detects endogenous level of ABL1/2 only when phosphorylated at tyrosine393/429. |
| Entrez Gene | 25 / 27 |
|---|---|
| Protein Accession | NP_005148.2 / NP_001129472.1 |
Application
Western blot analysis of lysed extracts from HL60 cells using ABL1/2 (phospho-Tyr393/429).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2 (Phospho-Tyr393/429). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/2 (phospho-Tyr393/429).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
