ABL2(ARG, Abelson-related gene) is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. The ABL2 gene product is expressed as two variants bearing different amino termini, both approximately 12-kb in length. c-Abl shows both cytoplasmic and nuclear localization,c-Abl is involved in two different chromosomal translocations present in human leukemias, which generate Bcr-Abl and TEL-Abl.1) Yoshimi I, Takashi I, Tsuneyuki O, et al. Blood. 2000; 95(6): 2126-2131.
Immunogen
Ni-NTA purified truncated recombinant ABL2 expressed in E. Coli strain BL21 (DE3).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
ELISAIFWB
Dilution Western Blot:1:200 - 1:1,000 IF:1:100 - 1:500 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Immunofluorescense analysis of ehtanol - fixed Hela cells showing cytoplasma staining using ABL2 antibody with IgG - FITC.
Application
Western blot analysis using ABL2 monoclonal antibody against truncated ABL2 recombinant protein.
Positive Control
1) Cat. No. 1201 - HeLa Cell Lysate
Application Notes
Dilution Western Blot:1:200 - 1:1,000 IF:1:100 - 1:500 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Additional Information
Form
Liquid
Storage Instructions
ABL2 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.