Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | ACER1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alkaline ceramidase 1, AlkCDase 1, Alkaline CDase 1, Acylsphingosine deacylase 3, N-acylsphingosine amidohydrolase 3, ACER1, ASAH3 |
| Product description | Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).1) Sun, W., et al. J. Invest. Dermatol. 128(2):389-397(2008) |
| Immunogen | This ACER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the C-terminal region of human ACER1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACER1 |
|---|---|
| Alternative Names | Alkaline ceramidase 1 AlkCDase 1 Alkaline CDase 1 Acylsphingosine deacylase 3 N-acylsphingosine amidohydrolase 3 ACER1 ASAH3 |
| Molecular Weight(MW) | 31 kDa |
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
