Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ACER3 Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | ACER3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alkaline ceramidase 3, AlkCDase 3, Alkaline CDase 3, 351-, Alkaline dihydroceramidase SB89, Alkaline phytoceramidase, aPHC, ACER3, APHC, PHCA |
| Product description | ACER3 hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.1) Wheeler,H.E., et.al., PLoS Genet.(10), E1000685 (2009) |
| Immunogen | This ACER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human ACER3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACER3 |
|---|---|
| Alternative Names | Alkaline ceramidase 3 AlkCDase 3 Alkaline CDase 3 351- Alkaline dihydroceramidase SB89 Alkaline phytoceramidase aPHC ACER3 APHC PHCA |
| Molecular Weight(MW) | 32 kDa |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ACER3 Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
