Application
Confocal immunofluorescent analysis of ACP1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of ACP1 Antibody in Hela cell line lysates (35ug/lane)| Product Name | ACP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1 |
| Product description | ACP1 belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios.1) Saccucci, P., et al. Med. Sci. Monit. 15 (10), CR511-CR517 (2009)Shu, Y.H., et al. J. Clin. Endocrinol. Metab. 94(10):4094-4102(2009) Apelt, N., et al. Metab. Clin. Exp. 58(10):1415-1423(2009) Banci, M., et al. Cardiology 113(4):236-242(2009) Rousseff, R.T., et al. Neuropediatrics 39(6):354-356(2008) |
| Immunogen | This ACP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human ACP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACP1 |
|---|---|
| Alternative Names | Low molecular weight phosphotyrosine protein phosphatase LMW-PTP LMW-PTPase Adipocyte acid phosphatase Low molecular weight cytosolic acid phosphatase Red cell acid phosphatase 1 ACP1 |
| Molecular Weight(MW) | 18 kDa |
Application
Confocal immunofluorescent analysis of ACP1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of ACP1 Antibody in Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
