Application
Western blot analysis of ACSM1 Antibody in K562 cell line lysates (35ug/lane)Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACSM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ACSM1 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy-and 2,3-or 3,4-unsaturated acids (in vitro). This protein functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase1) Celis,J.E., et.al., Mol. Cell Proteomics(10), 1795-1809 (2008) |
| Immunogen | This ACSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-48 amino acids from the N-terminal region of human ACSM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACSM1 |
|---|---|
| Alternative Names | Acyl-coenzyme A synthetase ACSM1 mitochondrial Acyl-CoA synthetase medium-chain family member 1 Butyrate--CoA ligase 1 Butyryl-coenzyme A synthetase 1 Lipoate-activating enzyme Middle-chain acyl-CoA synthetase 1 ACSM1 BUCS1 LAE MACS1 |
| Molecular Weight(MW) | 65 kDa |
Application
Western blot analysis of ACSM1 Antibody in K562 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
