ADAT1 is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, the family participates in the pre-mRNA editing of nuclear transcripts. ADAT1, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA.1) Venter, J.C., Science 291 (5507), 1304-1351 (2001).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ADAT1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
ADAT1 antibody can be used for detection of ADAT1 by ELISA at 1:62500. ADAT1 antibody can be used for detection of ADAT1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
ADAT1 antibody can be used for detection of ADAT1 by ELISA at 1:62500. ADAT1 antibody can be used for detection of ADAT1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ADAT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt