| Product Name | Adiponectin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACDC, ACRP30, APM1, GBP28, Adiponectin, 30 kDa adipocyte complement-related protein, ADPN, APM-1, ADIPQTL1 |
| Immunogen | Hi-5 Insect cells derived Recombinant Human Adiponectin |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADIPOQ |
|---|---|
| Alternative Names | ACDC ACRP30 APM1 GBP28 Adiponectin 30 kDa adipocyte complement-related protein ADPN APM-1 ADIPQTL1 |
| Application Notes | ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
