| Product Name | Adiponectin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa |
| Product description | ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels. |
| Immunogen | Recombinant human adiponectin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| ELISA: (direct and indirect: 1:5,000-1:10,000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ADIPOQ |
|---|---|
| Alternative Names | ACRP30 AdipoQ apM1 GBP28 Adipocyte Complement Related Protein of 30kDa |
| Tissue Specificity | Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested. |
| Entrez Gene | 9370 |
|---|---|
| Protein Accession | NP_001171271.1 |
| Application Notes | ELISA: (direct and indirect: 1:5,000-1:10,000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
