Application
Immunohistochemistry of ADROPIN in human brain tissue with ADROPIN antibody at 5 μg/mL.Application
Immunofluorescence of ADROPIN in human brain tissue with ADROPIN antibody at 20 μg/mL.Application
Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | ADROPIN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADROPIN Antibody: UNQ470, C9orf165, UNQ470/PRO830, Adropin, Energy homeostasis-associated protein |
| Product description | ADROPIN is a recently identified protein that has been implicated in the maintenance of energy homeostasis and insulin resistance (1-3). ADROPIN expression is regulated by energy status and dietary nutrient content and is altered by obesity and regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma (PPAR-gamma) (1). ADROPIN levels increase with dietary fat content (2). ADROPIN has also been proposed to play a role in the regulation of endothelial function (3).1) Kumar KG, Trevaskis JL, Lam DD, et al. Identification of Adropin as a secreted factor linking dietary macronutrient intake with energy homeostasis and lipid metabolism. Cell Metab. 2008; 8:468-81. |
| Immunogen | ADROPIN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ADROPIN. The immunogen is located within the last 50 amino acids of ADROPIN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENHO |
|---|---|
| Alternative Names | ADROPIN Antibody: UNQ470 C9orf165 UNQ470/PRO830 Adropin Energy homeostasis-associated protein |
| Molecular Weight(MW) | Predicted: 8 kDa Observed: 16 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | ADROPIN antibody is human, mouse and rat reactive. Despite its predicted molecular weight, ADROPIN often migrates at a higher than expected molecular weight in SDS-PAGE. |
Application
Immunohistochemistry of ADROPIN in human brain tissue with ADROPIN antibody at 5 μg/mL.
Application
Immunofluorescence of ADROPIN in human brain tissue with ADROPIN antibody at 20 μg/mL.
Application
Western blot analysis of ADROPIN in human brain tissue lysate with ADROPIN antibody at 2 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ADROPIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ADROPIN antibody is supplied in PBS containing 0.02% sodium azide. |
