Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PRAF1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000| Product Name | PRAF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRAF1 (polymerase (RNA) I polypeptide E, 53kDa)DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRAF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (100%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ13390 antibody FLJ13970 antibody FLJ43482 antibody PAF53 antibody PRAF1 antibody RP11-405L18.3 antibody POLR1E antibody DNA-directed RNA polymerase I subunit RPA49 antibody RNA polymerase I associated factor 53 antibody DNA-directed RNA polymer |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (3) |
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PRAF1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000| Positive Control | A549 , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
