Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000| Product Name | PRAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRAK (mitogen-activated protein kinase-activated protein kinase 5)The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRAK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Pig, Chicken, Rhesus Monkey, BovineMouse (98%), Rat (96%), Zebrafish (92%), Pig (98%), Chicken (95%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRAK antibody MAPKAPK5 antibody MAPK-activated protein kinase 5 antibody MAPKAP kinase 5 antibody MAP kinase-activated protein kinase 5 antibody MAPKAPK-5 antibody p38-regulated/activated protein kinase antibody mitogen-activated protein kinase-acti |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (3) |
| Cellular Localization | Cytoplasm (Probable) , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
