Application
Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody diluted at 1:1000.| Product Name | PRAS40 (phospho Thr246) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRAS40 (phospho Thr246) (AKT1 substrate 1 (proline rich))AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRAS40 (phospho Thr246). The exact sequence is proprietary. |
| Modification | p-Thr246 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Chimpanzee, BovineHuman (100%), Rat (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | PRAS40 (phospho Thr246) |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (5) |
| Cellular Localization | Cytoplasm , cytosol |
Application
Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody diluted at 1:1000.| Positive Control | NIH3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
