Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Akt1 S1 (Ab-246) Antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from 293 cells, using Akt1 S1 (Ab-246) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | PRAS40 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PRAS40Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Akt1 S1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | prolinerich Akt substrate 40 kDa Akt1 S1 Lobe proline-rich Akt substrate 40 kDa MGC2865 Akt1 substrate1 (prolinerich) Akt1 substrate 1 (proline-rich) Akt1 substrate 1 (prolinerich) PRAS40 prolinerich Akt substrate40 kDa |
|---|---|
| Target | PRAS40 |
| Molecular Weight(MW) | 27 kDa (note) |
| Tissue Specificity | Akt1 S1 Antibody detects endogenous levels of total Akt1 S1 protein. |
| Cellular Localization | Found in the cytosolic fraction of the brain. |
Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Akt1 S1 (Ab-246) Antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from 293 cells, using Akt1 S1 (Ab-246) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
