Application
AKT1S1 Antibody (Cat. #AM2018a) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the AKT1S1 antibody detected the AKT1S1 protein (arrow).| Product Name | AKT1S1 Antibody(Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 449CT13.4.2 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~4000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PRAS40 |
|---|---|
| Alternative Names | AKT1S1 PRAS40 Proline-rich AKT1 substrate 1 40 kDa proline-rich AKT substrate |
| Molecular Weight(MW) | 27383 Da |
| Function | Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection |
| Tissue Specificity | Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293) |
| Cellular Localization | Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity) |
| Entrez Gene | 84335 |
|---|---|
| Nucleotide Accession | NP_115751.2 NP_001092103.1 |
Application
AKT1S1 Antibody (Cat. #AM2018a) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the AKT1S1 antibody detected the AKT1S1 protein (arrow).| Application Notes | WB:1:500~4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
