Application
Immunofluorescence analysis of methanol-fixed A431, using PRC1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using PRC1 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: JurKat B: Raji 7.5% SDS PAGE diluted at 1:1000Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRC1 antibody [N1N2], N-term diluted at 1:1000.| Product Name | PRC1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRC1 (protein regulator of cytokinesis 1)This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PRC1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (81%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ASE1 antibody MGC1671 antibody MGC3669 antibody PRC1 antibody protein regulator of cytokinesis 1 antibody protein regulating cytokinesis 1 antibody anaphase spindle elongation 1 homolog antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) (4) |
Application
Immunofluorescence analysis of methanol-fixed A431, using PRC1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using PRC1 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: JurKat B: Raji 7.5% SDS PAGE diluted at 1:1000
Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRC1 antibody [N1N2], N-term diluted at 1:1000.| Positive Control | Jurkat , Raji , Mouse testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
