Application
Immunohistochemical analysis of PRDM2 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdPRDM2 , Adnull, or buffer alone were processed for PRDM2 in situ detection (TUNEL) analysis.Application
Rat B50 brain tumor cell extracts were immunoprecipitated with PRDM2 antibody or preimmune.| Product Name | PRDM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PRDM2Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a founding member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues. |
| Immunogen | Purified recombinant fusion protein, corresponding to amino acids 245-573 of Rat PRDM 2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| ELISA: Use at a dilution of 1/1000. IHC: Use at a dilution of 1/100 - 1/500. (Specific reference using this technique Cancer Res. 1998 Oct 158(19):4238-44)IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/100 - 1/500. Predicted molecular weight: 189 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Riz |
|---|---|
| Target | PRDM2 |
| Tissue Specificity | This antibody recognizes the beta isoform (Riz 2) of PRDM 2 (185 kDa) as well as RIZ 1. |
Application
Immunohistochemical analysis of PRDM2 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdPRDM2 , Adnull, or buffer alone were processed for PRDM2 in situ detection (TUNEL) analysis.
Application
Rat B50 brain tumor cell extracts were immunoprecipitated with PRDM2 antibody or preimmune.| Positive Control | Rat B50 brain tumour cell lysate |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/1000. IHC: Use at a dilution of 1/100 - 1/500. (Specific reference using this technique Cancer Res. 1998 Oct 158(19):4238-44)IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/100 - 1/500. Predicted molecular weight: 189 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS |
