Application
PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRDM4 protein stained by PRDM4 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using PRDM4 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using PRDM4 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG 7.5% SDS PAGE diluted at 1:5000Application
PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRDM4 protein stained by PRDM4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | PRDM4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRDM4 (PR domain containing 4)The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. An elevated expression level of this gene has been observed in PC12 cells treated with nerve growth factor, beta polypeptide (NGF). This gene is located in a chromosomal region that is thought to contain tumor suppressor genes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PRDM4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rabbit, Rhesus MonkeyRat (93%), Rabbit (92%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC45046 antibody PFM1 antibody PRDM4 antibody PR domain-containing protein 4 antibody PR-domain zinc-finger protein PFM1 antibody PR domain zinc finger protein 4 antibody PR domain containing 4 antibody |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRDM4 protein stained by PRDM4 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using PRDM4 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using PRDM4 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG 7.5% SDS PAGE diluted at 1:5000
Application
PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRDM4 protein stained by PRDM4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | PC-3 , U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
