Application
Immunocytochemistry of precerebellin in 3T3 cells with precerebellin antibody at 10 ug/ml.Application
Western blot analysis of precerebellin in 293 cell lysate with precerebellin antibody at (A) 2 and (B) 4 ug/ml.| Product Name | Precerebellin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PrecerebellinPrecerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q (1). Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway (2); and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release (3). The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus (4). Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin posseses functions other than those already stated (5). |
| Immunogen | Human Precerebellin / Cerebellin precursor / Cbln1 (C-Terminus) Peptide |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cbln1 Cerebellin precursor Precerebellin |
|---|---|
| Target | Precerebellin |
| Tissue Specificity | This antibody is specific for Precerebellin C-Terminus |
Application
Immunocytochemistry of precerebellin in 3T3 cells with precerebellin antibody at 10 ug/ml.
Application
Western blot analysis of precerebellin in 293 cell lysate with precerebellin antibody at (A) 2 and (B) 4 ug/ml.| Positive Control | Blocking Peptide: Cat. No. GTX85500-PEP - Precerebellin Peptide Lysates: Cat. No. GTX27902 - 293 Cell LysateCat. No. GTX27901 - 3T3 Whole Cell Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
