Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRICKLE3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:500| Product Name | PRICKLE3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRICKLE3 (prickle homolog 3 (Drosophila))LIM domain only 6 is a three LIM domain-containing protein. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface for protein-protein interactions. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRICKLE3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (98%), Rat (98%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LMO6 antibody PRICKLE3 antibody LIM domain only protein 6 antibody prickle-like protein 3 antibody triple LIM domain protein 6 antibody LMO-6 antibody prickle homolog 3 (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRICKLE3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:500| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
