Application
Western blot analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP antibody (clone EM-20) . CJD positive patient has proteinase K resistent prion protein. Lane 1, 4: Samples with proteinase K treatment Lane 2, 3: Samples without proteinase K treatment| Product Name | Prion Protein (PrP) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [EM-20] to Prion Protein (PrP)CD230 / Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K. Entrez Gene: 20p13 |
| Immunogen | Recombinant human prion protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | EM-20 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at 0.5 µg/mLNote:Non-reducing conditions are essential: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ASCR CJD GSS MGC26679 PRIP PrP PrP27-30 PrP33-35C PrPc Human Prion Protein PrP |
|---|---|
| Target | CD230 |
| Tissue Specificity | The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein. |
| Cellular Localization | Accumulates outside the secretory route in the cytoplasm, from where it relocates to the nucleus. |
Application
Western blot analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP antibody (clone EM-20) . CJD positive patient has proteinase K resistent prion protein. Lane 1, 4: Samples with proteinase K treatment Lane 2, 3: Samples without proteinase K treatment| Application Notes | WB: Use at 0.5 µg/mLNote:Non-reducing conditions are essential: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
