Application
at a 1mg/ml concentration staining~23 kDa (mature) and ~28 kDa (pro-peptide)native bovine prion proteinfrom brain (M. oblongata; 100 mg comprising 10% homogenised brain,diluted 1:10 in buffer, 10ml loaded) by Western blot (non-reducing conditions| Product Name | Prion protein PrP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1E5 / G6] to Prion protein PrPPrion diseases or transmissible spongiform encephalopathies are neurodegenerative diseases that affect both humans and animals (Prusiner, S. B.). All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPsc) (Pan, K. M. et al.). Both PrPsc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids(Basler, K. et al.). Many mammalian PrPs have a 22 amino acid N-terminal signal sequence (Hope, J. et al.; Turk, E. et al.) and 23 amino acid C-terminal signal sequence encoding for attachment of a glycosyl phosphatidylinositol anchor (Stahl, N. et al.). The mature protein of 209 amino acids contains one disulfide bond (Turk, E. et al.) and has two sites of asparagine-linked glycosylation (Endo, T. Et al.; Oesch, B. et al.). |
| Immunogen | Recombinant bovine prion protein. Immunization was performed according to the protocol described by Hofmann, J. et al. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E5 / G6 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: use at a concentration of 4 ng/ml. Western blot (non-reducing conditions): use at a concentration of 10 μg/ml. Optimal dilutions / concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Prion protein PrP PRNP PrP2730 PrP33-35C 5621 ASCR PrP3335C GSS PrP27-30 CJD PRIP PrPc PrP MGC26679 P04156 123400 |
|---|---|
| Target | Prion protein PrP |
| Tissue Specificity | This antibody detects:Human recombinant prion protein.Bovine recombinant, native and PrP res proteinase K treated prion protein. |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
Application
at a 1mg/ml concentration staining~23 kDa (mature) and ~28 kDa (pro-peptide)native bovine prion proteinfrom brain (M. oblongata; 100 mg comprising 10% homogenised brain,diluted 1:10 in buffer, 10ml loaded) by Western blot (non-reducing conditions| Application Notes | ELISA: use at a concentration of 4 ng/ml. Western blot (non-reducing conditions): use at a concentration of 10 μg/ml. Optimal dilutions / concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
