Application
IHC-P using| Product Name | Prion protein PrP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [7D9] to Prion protein PrPPrion protein PrP is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Two transcript variants encoding the same protein have been found for this gene. |
| Immunogen | This antibody was developed using a recombinant mouse PrP protein, aa 23-237. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7D9 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| ELISA: Use at a dilution of 1/20,000 - 1/50,000. IHC-P: Use at a dilution of 1/100 - 1/500. Use at an assay dependent dilution. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature or HIER (heat Induced Epitope Retrieval) before commencing with IHC staining protocol. WB: Use at a dilution of 1/10,000 - 1/25,000. Predicted molecular weight: 32 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseCatDeerElkHamsterSheepBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | Prion protein PrP PRNP PrP2730 PrP33-35C 5621 ASCR PrP3335C GSS PrP27-30 CJD PRIP PrPc PrP MGC26679 P04156 123400 CD230 prion |
|---|---|
| Target | Prion protein PrP |
| Tissue Specificity | recognises both protease sensitive and protease resistant forms ofPrP (after denaturing). |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
Application
IHC-P using| Application Notes | ELISA: Use at a dilution of 1/20,000 - 1/50,000. IHC-P: Use at a dilution of 1/100 - 1/500. Use at an assay dependent dilution. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature or HIER (heat Induced Epitope Retrieval) before commencing with IHC staining protocol. WB: Use at a dilution of 1/10,000 - 1/25,000. Predicted molecular weight: 32 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS |
