Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human breast lysate Lane 2: human kidney lysate Lane 3: human lung lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgApplication
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ADM Antibody in mouse lung tissue lysates (35ug/lane)Application
Western Blot at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ADM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADM, Adrenomedullin, AM, Proadrenomedullin N-20 terminal peptide, ProAM N-terminal 20 peptide, PAMP, ProAM-N20, ADM, AM |
| Product description | ADM, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes.1) Kim, S.M., et al. FEBS Lett. 584(1):213-218(2010) |
| Immunogen | This ADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-96 amino acids from the Central region of human ADM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:25: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADM |
|---|---|
| Alternative Names | ADM Adrenomedullin AM Proadrenomedullin N-20 terminal peptide ProAM N-terminal 20 peptide PAMP ProAM-N20 ADM AM |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human breast lysate Lane 2: human kidney lysate Lane 3: human lung lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig
Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ADM Antibody in mouse lung tissue lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:25: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
