Application
Western blot analysis of ADORA2B in mouse kidney tissue lysate with ADORA2B antibody at (A) 0.25 and (B) 0.5 μg/mLApplication
Immunofluorescence of ADORA2B in rat colon tissue with ADORA2B antibody at 20 μg/mL.Application
Immunohistochemistry of ADORA2B in rat colon tissue with ADORA2B antibody at 5 μg/mL.| Product Name | ADORA2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenosine receptor A2b, ADORA2 |
| Product description | The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).1) Pierce KD, Furlong TJ, Selbie LA, et al. Molecular cloning and expression of an adenosine A2b receptor from human brain. Biochem. Biophys. Res. Commun. 1992; 187:86-93. |
| Immunogen | ADORA2B antibody was raised against a 19 amino acid peptide near the amino terminus of human ADORA2B. The immunogen is located within the last 50 amino acids of ADORA2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADORA2B |
|---|---|
| Alternative Names | Adenosine receptor A2b ADORA2 |
| Molecular Weight(MW) | Predicted: 37 kDa Observed: 41 kDa |
| Sequence Similarities | 2) Cat. No. 1411 - Mouse Colon Tissue Lysate |
| Tissue Specificity | ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A. |
Application
Western blot analysis of ADORA2B in mouse kidney tissue lysate with ADORA2B antibody at (A) 0.25 and (B) 0.5 μg/mL
Application
Immunofluorescence of ADORA2B in rat colon tissue with ADORA2B antibody at 20 μg/mL.
Application
Immunohistochemistry of ADORA2B in rat colon tissue with ADORA2B antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ADORA2B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ADORA2B antibody is supplied in PBS containing 0.02% sodium azide. |
